Mineral chemistry and 3D tomography of a Chang'E 5 high-Ti basalt: implication for the lunar thermal evolution history.

In December 2020, Chang'E-5 (CE-5), China's first lunar sample return mission, successfully collected samples totaling 1731 g from the northern Oceanus Procellarum. The landing site was located in a young mare plain, a great distance from those of Apollo and Luna missions. These young mare basalts bear critical scientific significance as they could shed light on the nature of the lunar interior (composition and structure) as well as the recent volcanism on the Moon. In this article, we investigated a CE-5 basalt sample (CE5C0000YJYX065) using a combination of state-of-art techniques, including high resolution X-ray tomographic microscopy (HR-XTM), energy dispersive X-ray spectroscopy (EDS)-based scanning electron microscope (SEM), and electron probe microanalysis (EPMA) to reveal its 3D petrology and minerology. Our results show that this sample has a fine- to medium-grained subophitic texture, with sparse olivine phenocrysts setting in the groundmass of pyroxene, plagioclase, ilmenite and trace amounts of other phases. It has an extremely high ilmenite modal abundance (17.8 vol%) and contains a significant amount (0.5 vol%) of Ca-phosphate grains. The mineral chemistry is in excellent agreement with that of Apollo and Luna high-Ti basalts. The major phase pyroxenes also display strong chemical zoning with compositions following the trends observed in Apollo high-Ti basalts. Based on current data, we came to the conclusion that CE5C0000YJYX065 is a high-Ti mare basalt with a rare earth element (REE) enriched signature. This provides a rigid ground-truth for the geological context at the CE-5 landing site and clarifies the ambiguity inferred from remote sensing surveys.

A unique stone skipping-like trajectory of asteroid Aletai.

Meteoroids/asteroids could deposit energy to Earth during their entries, which arouses great concerns. Strewn field, as a product of meteoroids/asteroids breakup, comprehensively reflects the trajectory, dynamics, and physical properties of meteoroids/asteroids. It typically has a length of several to a dozen kilometers. Nevertheless, the recently found massive Aletai irons in the northwest China comprise the longest known strewn field of ~430 kilometers. This implies that the dynamics of Aletai could be unique. Petrographic and trace elemental studies suggest that all the Aletai masses exhibit unique compositions (IIIE anomalous), indicating that they were from the same fall event. Numerical modeling suggests that the stone skipping-like trajectory associated with a shallow entry angle (e.g., ~6.5° to 7.3°) is responsible for Aletai's exceptionally long strewn field if a single-body entry scenario is considered. The stone skipping-like trajectory would not result in the deposition of large impact energy on the ground but may lead to the dissipation of energy during its extremely long-distance flight.

An extraterrestrial trigger for the mid-Ordovician ice age: Dust from the breakup of the L-chondrite parent body.

The breakup of the L-chondrite parent body in the asteroid belt 466 million years (Ma) ago still delivers almost a third of all meteorites falling on Earth. Our new extraterrestrial chromite and 3He data for Ordovician sediments show that the breakup took place just at the onset of a major, eustatic sea level fall previously attributed to an Ordovician ice age. Shortly after the breakup, the flux to Earth of the most fine-grained, extraterrestrial material increased by three to four orders of magnitude. In the present stratosphere, extraterrestrial dust represents 1% of all the dust and has no climatic significance. Extraordinary amounts of dust in the entire inner solar system during >2 Ma following the L-chondrite breakup cooled Earth and triggered Ordovician icehouse conditions, sea level fall, and major faunal turnovers related to the Great Ordovician Biodiversification Event.

Cloning and characterization of enoate reductase with high ß-ionone to dihydro-ß-ionone bioconversion productivity.

BACKGROUND: Dihydro-ß-ionone is a principal aroma compound and has received considerable attention by flavor and fragrance industry. The traditional method of preparing dihydro-ß-ionone has many drawbacks, which has restricted its industrial application. Therefore, it is necessary to find a biotechnological method to produce dihydro-ß-ionone. RESULTS: In this study, the enoate reductase with high conversion efficiency of ß-ionone to dihydro-ß-ionone, DBR1, was obtained by screening four genetically engineered bacteria. The product, dihydro-ß-ionone, was analyzed by GC and GC-MS. The highest dihydro-ß-ionone production with 308.3 mg/L was detected in the recombinant strain expressing DBR1 which was later on expressed and purified. Its optimal temperature and pH were 45 °C and 6.5, respectively. The greatest activity of the purified enzyme was 356.39 U/mg using ß-ionone as substrate. In the enzymatic conversion system, 1 mM of ß-ionone was transformed into 91.08 mg/L of dihydro-ß-ionone with 93.80% of molar conversion. CONCLUSION: DBR1 had high selectivity to hydrogenated the 10,11-unsaturated double bond of ß-ionone as well as high catalytic efficiency for the conversion of ß-ionone to dihydro-ß-ionone. It is the first report on the bioconversion of ß-ionone to dihydro-ß-ionone by using enoate reductase.

High-level expression of recombinant thermostable ß-glucosidase in Escherichia coli by regulating acetic acid.

In the fermentation progress, fermentation parameters including the feed rate, induction temperature, and induction pH evidently regulate the accumulation of acetic acid generated by recombinant E. coli in the medium. The production of thermostable ß-glucosidase (Tpebgl3) was increased by optimizing the parameters mentioned step by step. The optimal conditions were obtained with the highest enzyme expression (560.4U/mL) and the maximum DCW (65g/L) at the pre-induction specific growth rate of 0.2h-1 followed by a post-induction specific growth rate (0.18h-1); induction temperature is 39°C; the pH is 7.2; the concentration of acetic acid was maintained all along below 0.9g/L. Results show it is necessary for the synthesis of Tpebgl3 to regulate the accumulation of acetic acid at the premise of feeding to meet the normal growth of E. coli. The production of Tpebgl3 by recombinant E. coli is the highest reported to date.